cox2 enzyme antibody  (Cell Signaling Technology Inc)

Journal: Regulatory peptides

Article Title: Increased circulating angiotensin-(1-7) protects white adipose tissue against development of a proinflammatory state stimulated by a high-fat diet.

doi: 10.1016/j.regpep.2012.06.009

Figure Lengend Snippet: Fig. 3. Adipose tissue inflammatory markers. Western blot for COX2-epididymal adipose tissue (A), RT-PCR expression of IL-1β‐epididymal adipose tissue (B), SD immunohisto- chemical reaction for IL-1β in epididymal adipose tissue (C), TGR immunohistochemical reaction for IL-1β in epididymal adipose tissue (D), RT-PCR expression of TNF-α‐ epididymal adipose tissue (E), SD immunohistochemical reaction for TNF-α in epididymal adipose tissue (F), TGR immunohistochemical reaction for TNF-α in epididymal adipose tissue (G). Data are presented as mean±SEM; * pb0.05.

Article Snippet: The blot was probed with COX2 enzyme antibody (Cell Signaling, USA; 1:1000), diluted in 1× blocking buffer, washed in 1× TBS with 1% of Tween 20, and then incubated with either IRDye 800 anti-goat secondary antibody (Rockland Immunochemicals®, Gilbertsville, Pa.) diluted to 1:10,000 in blocking buffer followed by further washes with PBS-Tween 20 and one wash in TBS.

Techniques: Western Blot, Reverse Transcription Polymerase Chain Reaction, Expressing, Immunohistochemical staining